Association of MicroRNA-146a expression level and it’s Gene Polymorphism rs2910164 C/G in patients with Rheumatoid Arthritis

MicroRNAs are small non-coding RNAs that can regulate a variety of immune functions. MicroRNA146a (miRNA-146a) is regarded as crucial regulator posttranscriptional gene expression, suggesting role in autoimmune disorders. This study was performed to investigate association miRNAs-146a expression and its polymorphisms patient with Rheumatoid arthritis. The current includes 120 subjects classified into two main groups: 60 RA patients healthy controls. Quantitative serum miRNA-146a, well genotyping rs2910164 (C/G) were done all using real-time PCR Tetra primer ARMS-PCR respectively. Serum miRNA-146a significantly over expressed (fold change 2.59±1.18), compared the controls 1.07±0.41), (P=< 0.0001).The receiver operating characteristics (ROC) curve plotted compare efficiency miR-146a showing high sensitivity 91.7 % specificity 91.1 %, AUC 0.95, (95% CI 0.91-0.99), cut off value (1.57). On other hand MiRNA-146a (rs2910164) revealed no significant variation between comparison control group under co-dominant model heterozygous genotype (C/G), homozygous (G/G), dominant pattern (C/G+G/G) genotypes, recessive (G/G) genotype. Analysis allele frequencies statistically differences Conclusion be used diagnostic markers for C/G not associated susceptibility. C considered protective.